Around 3pm on Thursday, a Pacific National goods train travelling south towards Adelaide collided with a Ford 4WD at a level crossing just south of Mallala.
The two occupants of the car were pronounced dead at the scene.
Major Crash Investigators have attended the scene of the collision and are investigating the circumstances surrounding the crash.
Anyone who witnessed the incident is asked to contact Crime Stoppers on 1800 333 000.
The two deaths brings the number of lives lost so far this year to 19, compared to 17 at the same time last year.
Emergency services are at the scene of a serious crash between a Pacific National freight train and a car at Mallala, about 60km north of Adelaide. Train was heading from Perth to Melbourne. Major crash investigators are making their way to the scene. More to come. @abcadelaide pic.twitter.com/iCwN7yGECS
— Shuba Krishnan (@ShubaSKrishnan) February 27, 2020