The duo were noticeably absent from the event held at Altum Restaurant in Milsons Point, Sydney, on Saturday.
In a massive group photo posted from the party, Knight and Gardner were nowhere to be seen, but fellow hosts, newsreader Tom Steinfort and entertainment reporter Brooke Boney, were in the snap.
Knight and Gardner hosted Today from January until November before being sacked.
Colleagues posted photos alongside Sydney Harbour at the swish event.
Channel 9 told news.com.au it would not comment on the stars’ absence because it was a private event.
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Gardner left the show last month after it was announced former host Karl Stefanovic would be returning to the failing program a year after he was dumped in favour of the duo.
With Gardner and Knight at the helm, ratings tumbled to embarrassing levels for Nine.
Knight and Steinfort remained on the show, but sports reporter Tony Jones also followed Gardner’s lead and quit early.
“I was consulted two weeks ago about the future direction of the Today show and, naturally, I’m disappointed with the outcome,” Gardner told The Sydney Morning Herald at the time.
Gardner was drafted to replace Lisa Wilkinson at the start of 2018 before Stefanovic was ousted that December.
Gardner’s farewell this time around was in stark contrast to the send-off she received in 2014.
After seven years as Today’s newsreader, Gardner announced in May 2014 that she had decided to leave the show to spend more time with her husband and two kids.
She returned in 2018 to take on the main hosting role.
Stefanovic will be back as host next year, alongside Allison Langdon.
Stefanovic and Gardner’s relationship suffered a very public blow only months into their on-air tenure last year when an Uber driver leaked the details of an explosive 45-minute conversation between Stefanovic and his brother Peter, a former Nine personality and now Sky News co-host.
The private phone call in March 2018 was reportedly heard by the Uber driver while Peter and his wife, Today newsreader Sylvia Jeffreys, were in the back of his car late at night, speaking to Karl on speakerphone.
According to the driver, Karl vented his frustration at Gardner, who he accused of “sitting on the fence” and not having enough opinions. The driver told New Idea magazine Karl became angry as he declared that she needed to “step up” if she “wanted to stay on the show”.