Premiers Richmond will again launch the season against Carlton in a Thursday night clash likely to attract more than 90,000 fans to the MCG.
Expectations are high for the Blues after they showed strong improvement in the second half of last season under David Teague.
Collingwood and the Western Bulldogs will face off on the Friday night at Marvel Stadium.
Essendon host Fremantle at the same venue on Saturday afternoon, Adelaide host Sydney and Gold Coast take on Port Adelaide at Metricon Stadium.
It was confirmed earlier in the week that GWS will host a Saturday night double-header against Geelong in both AFL and AFLW at Giants Stadium.
North Melbourne and St Kilda open proceedings on Sunday before Hawthorn host Brisbane in one of just two MCG games for the opening round.
AFL ROUND 1 2020 FIXTURE (all times AEDT)
* THURSDAY, March 19
Richmond v Carlton at MCG, 7.25pm
FRIDAY, March 20
Western Bulldogs v Collingwood at Marvel Stadium, 7.50pm
SATURDAY, March 21
Essendon v Fremantle at Marvel Stadium, 1.45pm
Adelaide v Sydney at Adelaide Oval, 4.35pm
GWS v Geelong at Giants Stadium, 7.25pm
Gold Coast v Port Adelaide at Metricon Stadium, 7.25pm
SUNDAY, March 22
North Melbourne v St Kilda at Marvel Stadium, 1.10pm
Hawthorn v Brisbane at MCG, 3.20pm
West Coast v Melbourne at Optus Stadium, 6.20pm